Table 1

Phenotypic characteristics of isolates from brine-lake sediments

L'Atalante
 Bannock
 Discovery
 Urania


AS2
 AS3
 AS5
 AS6
 AS7
 AS12
 AS28
 BS3
 BS6
 BS12
 BS17
 BS18
 BS25
 BS29
 DS1
 DS5
 DS9
 DS10
 DS16
 US1
 US2
 US4
 US7
 US13
 US16
Salinity of isolation medium [% NaCl]
 12
 12
 12
 3.5
 3.5
 3.5
 3.5
 3.5
 3.5
 3.5
 12
 12
 3.5
 3.5
 12
 3.5
 3.5
 3.5
 3.5
 3.5
 3.5
 3.5
 3.5
 12
 12
Max. NaCl [%]
 30
 30
 22
 15
 15
 10
 5
 15
 10
 10
 22
 25
 5
 10
 20
 10
 5
 10
 10
 15
 15
 15
 10
 30
 22
Growth at
























     4°C
 -
 -
 -
 -
 -
 +
 -
 -
 -
 -
 -
 -
 -
 -
 +
 +
 +
 +
 -
 +
 -
 -
 -
 -
 -
     45°C
 +
 +
 -
 +
 +
 -
 +
 +
 +
 -
 +
 +
 -
 +
 +
 +
 +
 +
 -
 +
 +
 +
 +
 +
 -
     53°C
 -
 -
 -
 -
 -
 -
 -
 +
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 +
 +
 -
 -
 -
     pH 5.5
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 -
 -
 +
 +
 +
 +
 -
 +
 +
 +
 -
 -
 -
     pH 6.5
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 -
 +
 +
 +
 +
 +
 -
 +
 +
 +
 +
 -
 +
     pH 9.0
 +
 +
 +
 +
 +
 +
 -
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
Fermentation1)
 +
 +
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 +
 +
 -
 -
 -
Motility
 +
 +
 +
 +
 +
 +
 -
 +
 +
 +
 +
 +
 +
 -
 +
 +
 -
 +
 +
 +
 +
 +
 -
 +
 +
Enzyme activity
























     Polymer degr.2)
 2
 2
 4
 4
 4
 2
 3
 4
 3
 2
 3
 2
 0
 5
 5
 4
 4
 3
 1
 4
 6
 6
 5
 2
 3
     Nitrite prod.
 -
 -
 -
 -
 -
 +
 +
 -
 -
 -
 -
 +
 +
 +
 +
 +
 -
 -
 -
 -
 +
 +
 +
 -
 +
     β-Galactosidase
 +
 +
 +
 +
 +
 +
 +
 -
 +
 +
 -
 +
 +
 +
 +
 +
 -
 +
 -
 -
 +
 +
 +
 -
 +
     Arginine dehyd.
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 -
 +
 -
 -
 -
 -
 -
 -
 -
 +
 +
 -
 -
 -
     Urease
 -
 -
 -
 -
 -
 -
 +
 -
 -
 -
 -
 +
 -
 -
 -
 -
 -
 +
 +
 -
 +
 -
 -
 -
 -
     Acetoin prod.
 -
 -
 -
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 -
     Catalase
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 -
 +
 +
 +
 -
 +
 +
 +
 +
 +
 -
     Oxidase
 -
 -
 -
 -
 -
 +
 +
 -
 -
 +
 +
 +
 -
 -
 -
 -
 +
 -
 -
 +
 +
 -
 -
 +
 -
Substrate utilization3)
























     Poly- and disaccharides (8)
 5
 5
 5
 7
 6
 5
 6
 5
 6
 6
 6
 6
 6
 6
 0
 0
 6
 5
 3
 6
 4
 4
 6
 4
 6
     Monosaccharides (14)
9
 9
 5
 6
 8
 9
 8
 9
 5
 9
 10
 9
 6
 8
 3
 1
 12
 13
 11
 10
 12
 12
 9
 9
 10
     Alcohols (5)
 1
 1
 0
 1
 1
 1
 1
 1
 1
 1
 2
 1
 1
 1
 1
 1
 5
 3
 0
 1
 1
 1
 1
 1
 1
     Fatty acids (4)
 0
 0
 0
 0
 1
 0
 1
 0
 0
 0
 2
 1
 0
 1
 0
 0
 2
 1
 0
 2
 0
 0
 4
 0
 0
     Carboxylic acids (7)
 5
 5
 0
 5
 6
 0
 3
 4
 5
 2
 6
 7
 0
 7
 1
 1
 7
 7
 6
 4
 6
 4
 7
 6
 0
     Amino acids (20)
 7
 7
 0
 8
 6
 0
 4
 5
 3
 2
 9
 0
 7
 3
 0
 0
 5
 8
 5
 7
 4
 4
 6
 4
 7
     Betaine, Salicylate, benzoate
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 2
 2
 0
 0
 0
 0
 0
 1
 0
 0
 0
 0
 0
 0
 1
     Alkanes (2)
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0
 2
 0
 2
 0
 0
 0
 0
 0
 0
 0
 0
 0
 0

1) Fermentation: fermentative growth with yeast extract. Note that while strain US13 did not grow by fermentation, it grew using dimethylsulfoxide or trimethylamine N-oxide as electron acceptor.

2) Number of positive results for polymer hydrolysis out of seven different tests; all strains used the complex substrates yeast extract, casamino acids and bactopeptone; all strains tested positive for tryptophan deaminase; and all strains tested negative for N2 production from nitrate, lysine decarboxylase, ornithine decarboxylase, H2S production, indole production. Degr. = degradation, prod. = production, dehyd. = dehydrogenase.

3) Numbers in brackets indicate the number of tested compounds in the respective category (see Methods for details of substrates tested); none of the isolates used serine, leucine, methionine or tyrosine. All isolates grew with yeast extract, peptone and casamino acids.

Sass et al. Saline Systems 2008 4:8   doi:10.1186/1746-1448-4-8