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PCR primers Primers used in PCR for constructing deletions and for screening uvr alleles. All listed 5'→3'. Engineered restriction sites underlined |
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| Primers for constructing deletions: |
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| uvrA1 forward |
GGGGGTACCGTATTTGTTCGGCACGAGGT |
| uvrA1 reverse |
GGGTCTAGACTCTTCGCTCATTGGGAGAG |
| uvrA2 forward |
GGGTCTAGACTCTCGCGGCTCTGTCTC |
| uvrA2 reverse |
GGGAAGCTTACCGTCTCAGTGGTGGTGTC |
| uvrB1 forward |
GGGGGTACCAGGAACGCGACCACTACG |
| uvrB1 reverse |
GGGTCTAGAGCTGGCGTCACTCATTACAC |
| uvrB2 forward |
GGGTCTAGAGAAACCGAGGACTGGTGAGA |
| uvrB2 reverse |
GGGAAGCTTGGGAACACGAAGATGAGGAA |
| uvrC1 forward |
GGGGGTACCGTACGTGGGTGTGATGAACG |
| uvrC1 reverse |
GGGTCTAGATTCACGACTGTCTCCACGTC |
| uvrC2 forward |
GGGTCTAGAAGAACGACGACTACGCGAAC |
| uvrC2 reverse |
GGGAAGCTTACGTCTCGGAGTACCAGCAG |
| Primers for screening deletions by PCR |
|
| uvrA up |
AATGTCGTAGTCGGCCATGT |
| uvrA down |
CACAGCCCCGAGACAGAG |
| uvrB up |
GGCCTACGACGAGTACACC |
| uvrB down |
TGAAAAGCGTTGGTTTCTCC |
| ura3 up |
CTTCCGGAGGACGTACAGG |
| ura3 down |
CGTACTGGGCGTTCCACT |
| bla up |
TTTGCCTTCCTGTTTTTGCT |
| bla down |
TTGCCGGGAAGCTAGAGTAA |
Crowley et al. Saline Systems 2006 2:11 doi:10.1186/1746-1448-2-11 |
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