Figure 4.

Halobacterium uvrA, uvrB, uvrC and uvrAuvrC mutants are completely deficient in dark repair of UV-induced photoproducts. (A) Sample dot blots (in duplicate) of total damage (CPDs and 6–4PPs, top) and 6-4PPs alone (bottom) for DNA samples isolated at the indicated times following 150 J/m² UV-C treatment to DJC501 (uvr⁺), DJC519 (uvrA), DJC520 (uvrB), DJC502 (uvrC) and DJC509 (uvrAuvrC). (B) Total damage and 6-4PPs from uvr⁺ and uvrA mutant strains during 3 hours post-UV incubation, showing repair in the wild-type and no repair in the mutant.